全文获取类型
收费全文 | 2244篇 |
免费 | 154篇 |
国内免费 | 131篇 |
专业分类
林业 | 202篇 |
农学 | 228篇 |
基础科学 | 24篇 |
127篇 | |
综合类 | 760篇 |
农作物 | 137篇 |
水产渔业 | 35篇 |
畜牧兽医 | 465篇 |
园艺 | 480篇 |
植物保护 | 71篇 |
出版年
2024年 | 3篇 |
2023年 | 44篇 |
2022年 | 72篇 |
2021年 | 78篇 |
2020年 | 93篇 |
2019年 | 91篇 |
2018年 | 69篇 |
2017年 | 107篇 |
2016年 | 118篇 |
2015年 | 110篇 |
2014年 | 138篇 |
2013年 | 173篇 |
2012年 | 175篇 |
2011年 | 160篇 |
2010年 | 124篇 |
2009年 | 113篇 |
2008年 | 144篇 |
2007年 | 122篇 |
2006年 | 86篇 |
2005年 | 58篇 |
2004年 | 50篇 |
2003年 | 49篇 |
2002年 | 48篇 |
2001年 | 39篇 |
2000年 | 43篇 |
1999年 | 22篇 |
1998年 | 16篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 17篇 |
1994年 | 20篇 |
1993年 | 15篇 |
1992年 | 17篇 |
1991年 | 14篇 |
1990年 | 19篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 1篇 |
1974年 | 2篇 |
排序方式: 共有2529条查询结果,搜索用时 62 毫秒
31.
我国受重金属污染的土地面积广大,因采矿等造成的环境和土壤重金属污染问题日趋严重。因金属矿床形成特点及开采方式单一等原因,造成Cd、Pb居于重金属复合污染前列。通过总结单一Cd、Pb污染对植物生理生化特征的影响以及Cd、Pb复合污染对植物生理生化及细胞结构的影响等研究进展,明晰土壤重金属Cd、Pb复合污染对植物影响和损害的研究热点,进而探明植物重金属胁迫的生理响应机制。 相似文献
32.
AIM: To investigate the effect of hesperetin on hypoxia/reoxygenation (H/R)-induced apoptosis in the H9c2 cells and to clarify the underlying mechanism. METHODS: The H/R model was established and the H9c2 cells were pretreated with hesperetin for 4 h. The cell viability and cell damage were measured by CCK-8 assay and lactate dehydrogenase (LDH) detection. The apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. The intracellular calcium fluorescence intensity was measured by fluorescence microscopy and flow cytometry. The calcium-ATPase activity and the level of adenosine triphosphate (ATP) were measured by ELISA. The mitochondrial membrane potential was measured by JC-1 staining. The protein expression levels of Bcl-2, Bax and cytochrome C (Cyt-C) were determined by Western blot. RESULTS: Hesperetin reduced the apoptosis of the H9c2 cells induced by H/R, decreased intracellular Ca2+ fluorescence intensity, elevated Ca2+-ATPase activity, inhibited the mitochondrial membrane potential depolarization and increased the level of ATP (P<0.05). In addition, hesperetin significantly reduced the release of Cyt-C protein from mitochondria to cytoplasma and increased the Bcl-2/Bax ratio (P<0.05). After using the calcium ion inhibitor nimodipine, the percentage of the cells with mitochondrial membrane depolarization was decreased, the ATP level was increased and the protein expression of mitochondrion-related apoptosis molecules were decreased (P<0.05). CONCLUSION: Hesperetin reduces the apoptosis of the H9c2 cells induced by H/R, which may be related to inhibition of calcium overload and improvement of mitochondrial function. 相似文献
33.
Atle Foss Hans van de Vis Wout Abbink Bert Lambooij Bjørn Roth 《Journal Of Applied Aquaculture》2019,31(1):34-47
In the present study, selected temperature drops were examined to investigate the effects of live chilling on stress and welfare in turbot and sole. This study demonstrated that rapid temperature drops from 11°C–12°C and 18°C–18.7°C to 4°C–0°C in turbot resulted in higher blood sodium and potassium at 0°C (164 mmol l?1, 4.4 mmol ?1) compared to 4°C (153 mmol l?1, 3.1 mmol ?1), indicating osmoregulatory disturbance. A rapid temperature drop from 18°C to 0°C in Senegal sole also resulted in higher blood sodium and potassium at 0°C (164 mmol l?1, 4.8 mmol ?1) compared to the control group at 18°C (157 mmol l?1, 3.2 mmol ?1). Based on present findings, we conclude that immersion in ice water will have a negative effect on the animal osmoregulatory capacity, and we recommend that turbot and sole are stunned before slaughter. 相似文献
34.
The effect of 0, 0.05 or 0.1 mm abscisic acid treatment on chilling tolerance and salicylic acid‐related responses was investigated in young maize seedlings (Zea mays L., hybrid Norma). Although the pre‐treatment of maize seedlings with abscisic acid slightly decreased the chlorophyll content, it also reduced the level of chilling injury caused by 6 days of cold treatment at 5 °C. Under normal growth conditions, increased levels of bound salicylic acid and of bound ortho‐hydroxycinnamic acid were observed in the leaves during abscisic acid treatment. In the roots, abscisic acid did not affect the free and bound salicylic acid levels, but increased the amount of free and bound ortho‐hydroxycinnamic acid. The activity of glutathione‐S‐transferase increased on the 3rd day of abscisic acid treatment, whereas it did not change when followed by cold stress, compared with the control leaves. In the roots, the activities of glutathione reductase, glutathione‐S‐transferase and ascorbate peroxidase increased during the abscisic acid treatment, and those of glutathione‐S‐transferase and ascorbate peroxidase were also stimulated when abscisic acid pre‐treatment was followed by cold stress, compared with the control roots. Our results suggest that an overlap may exist between the abscisic acid‐induced cold acclimation and the salicylic acid‐related stress response. 相似文献
35.
为探讨草酸处理对低温冷藏下芒果冷害及细胞壁代谢的影响,本试验以桂七芒果果实为试材,采用5 mmol·L-1草酸溶液浸泡处理,以清水浸泡处理为对照,并于4℃贮藏,分析芒果的冷害指数、丙二醛(MDA)含量、相对电导率、硬度、细胞壁物质含量、细胞壁代谢酶的变化。结果表明,与对照组相比,草酸处理显著降低了低温贮藏14 d后桂七芒果果实冷害指数、MDA含量、相对电导率、原果胶和纤维素含量,显著降低了低温贮藏28 d后果实的硬度;显著提高了低温贮藏14 d后果实水溶性果胶含量及多聚半乳糖醛酸酶(PG)、果胶甲酯酶(PME)、纤维素酶(Cx)活性,显著提高了贮藏21 d后果实β-半乳糖苷酶(β-Gal)活性。综上所述,草酸处理能减轻桂七芒果冷害,维持采后果实细胞壁降解酶较高活性和水溶性果胶含量。本研究为揭示草酸减轻芒果果实冷害机制提供了依据,可为草酸应用于其他冷敏型果实的贮藏保鲜提供理论参考。 相似文献
36.
热处理对低温胁迫下黄瓜活性氧代谢和膜脂组分的影响 总被引:1,自引:0,他引:1
为探讨热处理减轻黄瓜果实低温冷害的作用机理,本试验研究了47℃热水浸泡5 min处理对黄瓜果实在4℃贮藏期间活性氧代谢和膜脂组分的影响。结果表明,47℃热水5 min处理可显著抑制黄瓜果实冷害的发生,贮藏15 d后果实冷害指数较对照(CK)低20.87%。此外,热处理还可抑制黄瓜相对电导率及丙二醛(MDA)含量的上升,提高黄瓜果实超氧化物歧化酶(SOD)、过氧化物酶(DOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性,抑制超氧阴离子($\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$)及过氧化氢(H2O2)的产生,同时降低脂氧合酶的活性,保持较低的饱和脂肪酸含量、较高的不饱和脂肪酸含量和膜脂不饱和度。综上,热处理可维持黄瓜果实活性氧代谢的平衡,抑制膜脂的过氧化作用,从而提高黄瓜果实的抗冷性,减轻果实冷害损伤。 相似文献
37.
廖华晓 《上海交通大学学报(农业科学版)》2014,(6):95-98
为了了解核心力量训练对普通非专业运动员预防运动损伤的作用,采用独立样本T检验(Independent Samples T Test)对实验组和对照组的FMS功能性测试成绩进行统计分析,实验结果显示核心力量训练对普通高校非专业运动员的平衡、稳定、协调能力有良好的促进作用;为四肢的运动传递能量,促使躯体保持平衡,提高身体在不稳定的状态下平衡稳定能力,减少运动损伤的发生机率,是一项简单易操作的训练手段。 相似文献
38.
39.
采用问卷调查、数理统计、文献资料等方法对我国滑翔伞运动损伤的情况及原因进分析。结果表明,由于滑翔伞运动本身的特点及此项运动在我国开展逐年广泛,损伤率较高,达到75%。其影响因素主要有参与者技术不够娴熟、场地因素和天气因素三方面。并根据调查结果提出预防措施。 相似文献
40.